Click on the speaker to read title and abstract for their lecture (updates continuously).
Keynote speaker: Mark R Wiesner
Mark R Wiesner is a James B. Duke Professor of Civil and Environmental Engineering at Duke University, USA. Wiesner's research interests include membrane processes, nanostructured materials, transport and fate of nanomaterials in the environment, colloidal and interfacial processes, and environmental systems analysis.
Nanomaterials : Not the next asbestos
Some lessons learned from 20 years of nanomaterial implications research
Nanomaterials exhibit novel, size-dependent properties that enable new applications ranging from molecular electronics to energy production. An early concern, expressed nearly 20 years ago, was that the novel properties associated with size might also translate into previously un-recognized modes of toxicity to organisms and ecosystems that might be generalized to the entire class of “nanomaterials.” Exposure to nanomaterials may arise during nanomaterial fabrication, handling of nanomaterials in subsequent processing to create derivative products, product usage, and as the result of post-usage or waste disposal practices. Hazards suggested for nanomaterials included that of the Trojan horse effect (conventional contaminants carried by nano-scale materials), alterations in protein configuration, oxidative stress, redox reactions, and others. The quantities of nanomaterials produced per year are large and the number of products incorporating nanomaterials has grown rapidly. However, apart from work-place exposures, these quantities are miniscule compared with exposures to natural and incidental nano-scale materials. While still controversial, research over the last 20 years has not yielded evidence for a uniquely nano-based pathway for toxicity. Individual types nanomaterials have been shown to exhibit toxicity, such as in the case of heavy-metal derived nanoparticles. Nanoscale formulations of these materials, previously known to be toxic in their bulk form, may yield dose-response curves that differ from their bulk counterparts. However, evidence is lacking for nano-based phenomena that produce toxicity in a general sense that would merit broad-brush regulation of nano-scale materials. This presentation briefly reviews the evidence for nanomaterial toxicity, considers the relative exposures to nano-scale materials and presents some the attributes of nanomaterial behavior in natural systems that have been described over the last 20 years.
Harri Alenius is a Professor of Molecular Toxicology at Karolinska Institutet. He is investigating how different environmental exposures, including environmental and endogenous microbes, nanomaterials and chemicals, affect human wellbeing. The aim of the research is to uncover the mechanisms behind the factors linking environmental exposures with immune tolerance and the breaking of immune tolerance. Harri Alenius has been a guest or visiting Professor at several universities, including Zhejiang University in China in 2015.
From conventional in vitro and in vivo approaches towards predictive nanotoxicology
Abstract: Current toxicological paradigms rely both on in vitro and in vivo exposure setups that often are difficult to compare, resulting in questioning the real efficacy of cell models to mimic more complex exposure scenarios at the organism level. Furthermore, experimental differences in the exposure procedures even between in vivo experiments have been challenged since some protocols, although mimicking real-life exposure scenarios, require special methodological and technological requirements and the need of high amounts of ENM. Simplified in vivo exposure procedures do not require expensive laboratory exposure equipment and require only a minute amounts of ENM - but these methods have been criticized since ENM must be introduced to the test organism in a dispersed form.
Utilization of systems biology approaches in the development of predictive toxicology provides possibilities to generate new and highly improved risk assessment paradigm. There is a need for clear understanding of the relationship between ENM characteristics and how these features can evoke biological responses in living organisms and cells. The computational based hazard prediction tools that are validated against state-of-the-art in vitro and in vivo toxicological methods would permit the crucial transition from descriptive toxicology to predictive toxicology.
In this presentation, the applicability of systems biology approaches to link conventional in vivo and in vitro approaches for predictive nanotoxicology will be discussed.
Ulla Vogel is Professor in Chemical Working Environment at the National Research Centre for the Working Environment (NFA) and Adjunct Professor in Nanosafety at the Technical University of Denmark. She is head of Danish Centre for Nanosafety and has worked with the toxicology of inhaled (nano)particles for 20 years with focus on cancer, cardiovascular disease and reprotoxicity. The Nanosafety research group at NFA is past and present partner in more than 20 EU projects related to nanosafety.
Health effects of Inhaled Nanomaterials
Abstract: Most nanoparticles are more hazardous (by mass) by inhalation compared to larger particles with the same chemical composition. This is especially true for nanoparticles with low solubility and low toxicity. Carbon nanotubes constitute a group of highly toxic nanomaterials when inhaled and other high-aspect-ratio nanomaterials may potentially have similar toxicity. Inhalation of nanomaterials has been shown to cause inflammation, acute phase response, fibrosis and tumors, thus linking inhalation of nanomaterials to risk of cardiovascular disease and cancer. Based on animal studies, NIOSH (National Institute of Occupational Safety and Health in the USA) suggested occupational exposure limits of 0.001 mg/ for carbon nanotubes and 0.3 mg/m3 for nanosized titanium dioxide.
In conclusion, inhalation of nanomaterials can be linked to risk of cancer and cardiovascular disease. Accordingly, occupational exposure to nanomaterials should be carefully assessed.
Professor Anna Ledin is Director for the Environmental Administration in the city of Gothenburg. She is also board member of the Mistra Environmental Nano Safety program board. She has been professor in environmental chemistry at DTU, KTH, LiU and LU and has for instance worked on characterization of nanoparticles in natural water systems in her PhD long time ago.
Lena Palmberg, MD is a Professor in Toxicology at Karolinska Institutet. The long term goal with her research is to lay the foundation for effective, future treatments through better understanding of the mechanisms behind chronic bronchitis and COPD (Chronic Obstructive Pulmonary Disease). Her research is also providing new knowledge on environmental factors that cause these diseases including exposure to particles/nanoparticles. She has developed advanced multicellular lung mucosa models including human primary cells as an important tool in her research. (Photographer: Ulf Sirborn).
Multi-cellular human lung models for toxicity testing
Abstract: It is important to develop and evaluate physiologically relevant lung mucosa models including both bronchial and alveolar mucosa models with primary human cells for assessing nanoparticles related health hazards. To develop these sophisticated airway wall models further, they can be co-cultured with fibroblast/endothelial cells and/or innate effector cells (macrophages) which not only mimic the in vivo-situation but also avoid the constant concern of species differences when using animal models. These models with multiple cell types enable us to study cell-to cell interactions and cross-talk between the cells. New approaches regarding exposure methods are needed to be able to expose the models cultured at air-liquid interface. Further, both acute and repeated exposures are important to mimic both acute and more chronic exposure situation. The models can be used to explore the interaction of exposure, therapeutic effects, innate immunity, protease/antiprotease balance and oxidative stress as well as the interactions of various cell types. Validation of the models will make it possible to develop a systematic in vitro-testing strategy in order to reduce the requirement for animal inhalation studies.
Mónica João de Barros Amorim
Mónica João de Barros Amorim is a Principal Investigator in Stress Biology at University of Aveiro in Portugal. She was the president of SETAC Europe (2014-2015). Present research area is soil nanotoxicology and exotoxicogenomics including the development of new microarray technologies.
Environmental hazards of NMs – potential (and challenges) of integrating omics
Abstract: Nanomaterials (NMs) present novel/added challenges to the standard effect assessment requiring an update of used methods. One way forward is to link sub organismal high-throughput (HTP) based responses to population outcomes, because effects on organisms are preceded by earlier changes on the sub-organismal level (cell, genes). This can allow for a rapid detection of effects during shorter exposure time and an understanding of the mechanisms of toxicity, towards a systems toxicology approach. Moreover, given the link, such data can be integrated onto Adverse Outcome Pathways (AOPs) and support a knowledge based risk assessment among others. Finally this approach also allow for detection of population effect in field organisms.
High-throughput omics endpoints like transcriptomics, metabolomics or proteomics offer support for the mechanistic understanding of stressors such as pollutants, including nanomaterials (NM) or any other novel materials. Case study(ies) will be given, where the mechanisms of toxicity were investigated using a suite of (novel) tools for environmental relevant organisms.
Last, we highlight main study gaps and list priority needs and the way forward.
Bengt Fadeel, M.D., Ph.D., is a Professor of Inflammation Research at the Institute of Environmental Medicine (IMM), Karolinska Institutet. He is an expert on toxicity assessment of engineered nanomaterials, with particular focus on immune effects. Prof. Fadeel also serves as the chair of the scientific expert panel of the national nanosafety platform, SweNanoSafe.
Cytotoxicity assessment of metal and metal oxide nanoparticles: lessons from nanosafety projects
Abstract: The increasing production and use of engineered nanomaterials (ENMs) has led to concerns about their potential adverse effects on human health and the environment. In particular, interactions of ENMs with immune cells are of considerable importance as the immune system represents the first line of cellular defense against foreign intrusion. The inflammogenic potential of metal and metal oxide nanoparticles has been the subject of extensive investigations in recent years [Bhattacharya et al. 2014]. However, a detailed understanding of the mechanism(s) underlying the toxicity of ENMs is still lacking. The application of systems biology approaches based on omics technologies coupled with computational analysis to elucidate perturbations of genes or proteins are being applied in nano(eco)toxicological research in order to develop predictive models of ENM toxicity in biological systems [Costa and Fadeel, 2016]. Our laboratory has been engaged in several European Commission-funded nanosafety projects in recent years including FP7-NANOMMUNE, FP7-MARINA, FP7-NANOREG, FP7-SUN, and FP7-NANOSOLUTIONS and we have screened a large number of ENMs including metal and metal oxide nanoparticles using primary human immune-competent cells or cell lines. Further studies were conducted in the national MISTRA Environmental Nanosafety project. In addition, we have applied transcriptomics and proteomics approaches to probe cellular and tissue responses to ENMs. In the present lecture, some of the lessons learned from these projects are discussed.
Bhattacharya K, Farcal L, Fadeel B. (2014). Shifting identities of metal oxide nanoparticles: focus on inflammation. MRS Bulletin. 39:970-5.
Costa PM, Fadeel B. (2016). Emerging systems biology approaches in nanotoxicology: towards a mechanism-based understanding of nanomaterial hazard and risk. Toxicology & Applied Pharmacology. 299:101-11.
Govind Gupta is a Postdoc at Institute of Environmental Medicine (IMM), Karolinska institutet. He has expertise in the safety assessment of nanomaterials in human in vitro cell line models including understanding their mechanisms of toxicity. He also has interest in nano-eco-interactions and fate assessment of nanomaterials in environmental systems.
Toxicity screening of a panel of silica nanoparticles: role of size and surface properties
Abstract: Silica (SiO2) nanoparticles are produced for a variety of applications and the amorphous form of silica are accepted as safe for use in food products by the US Food and Drug Administration. However, experimental studies in recent years have raised concerns regarding the safety of certain SiO2 nanoparticles (Shi et al. ACS Nano. 2012;6(3):1925-38; Zhang et al., J Am Chem Soc. 2012;134(38):15790-804). In the present study, we investigated a panel of SiO2 nanoparticles of different sizes (7 nm to 100 nm) and different surface modifications using the human THP-1 cell line, and we found that ultrasmall (7 nm) nanoparticles showed the strongest cytotoxic effect. Surface modification of the particles with ethoxy-silane completely diminished the cytotoxicity of the SiO2 particles. Further analysis of the mechanism of cytotoxicity of the ultrasmall nanoparticles revealed that lipid peroxidation and autophagy were involved in SiO2 nanoparticle-triggered cell death. Transmission electron microscopy showed that the unmodified nanoparticles were attached to the cell surface and studies using FITC-labeled SiO2 nanoparticles confirmed this observation and also showed that a fraction of the particles were co-localized with lysosomes. These studies suggest that ethoxy-silane doping may be considered as a ‘safe-by-design’ approach for SiO2 nanoparticles. This work was supported by the MISTRA Environmental Nanosafety project.
Tommy Cedervall is an Associate Professor at Dept. of Biochemistry and Structural Biology, Lund University. He has expertise in the interactions between biomolecules, mostly proteins and nanomaterials. Experienced in the characterization of the identity of bound proteins in biological fluids, the affinity between protein and nanomaterials, the stoichiometry, and structure and function of bound proteins.
Aggregation of nanoplastics and other nanomaterials in ecotoxicological studies
Abstract: It is well known that nanomaterials, including nanoplastics, upon contact with the environment interacts with biomolecules creating what is often called a biocorona. The biocorona will affect the characteristics of the nanomaterial. In the experimental set ups in commonly used ecotoxicological tests, the nanoparticles are expected to aggregate due to changes in pH, salinity, the presence of biomolecules, and the interaction with the test organism itself. Differences in the biocorona will affect the interactions with the test organism as well as the dispersion stability. The effect will depend on the molecular content of the biocorona, the ratio of biomolecules and nanoparticles and the concentration of the stock solution of nanoparticles. Furthermore, different conditions will result in aggregates with different stability and possibly morphology. Using nanoplastics, and gold and titanium oxide nanoparticles, we have started to investigate the complex relationship between nanoparticles, surrounding environment, aggregate formation, and the biological effect.
Mikael Ekvall is a researcher at Biochemistry and Structural Biology, Lund University. Mikael has a PhD in biology and his expertise is aquatic ecology. Mikael's research focus on effects of nanomaterials on freshwater organisms and trophic transfer of nanomaterials.
Long-term effects of tungsten carbide (WC), tungsten carbide cobalt (WC-Co) and cobalt (Co) nanoparticles on Daphnia magna and the effect of an environmental corona.
Abstract: Tungsten carbide (WC) and tungsten carbide cobalt (WC-Co) is widely used for its hard metal properties, although its use, in for instance tyre studs, may result in nano-sized particles ending up in nature. We evaluated the long-term effect of exposing a freshwater zooplankton (Daphnia magna) to tungsten carbide NPs. Despite not being acutely toxic we show that long-term exposure can result in both increased time to first reproduction as well as a reduction in survival time if the particles are allowed to be resuspended. We also show that treating the particles with a relevant environmental corona results in a reduction in toxicity.
Inger Odnevall Wallinder
Inger Odnevall Wallinder is a Professor at the Division of Surface and Corrosion Science at KTH Royal Institute of Technology. She has more than 20 years expertise in the characterization of surface and bulk properties of nano- and micron-sized metallic particles, surface reactivity, surface interactions with biomolecules, metal release and speciation in biological media, and links to toxicity, as well as colloidal stability and mobility and environmental fate of nanoparticles.
Finding linkages between toxicity and surface reactivity of metallic nanoparticles
Abstract: From the rapidly emerging use of metallic NPs in different applications in the society follows risks for their potential diffuse environmental dispersion and potency to induce adverse effects on both humans and the environment. Even though it is well-known that metal-containing particles can be harmful, underlying mechanistic reasons are not well understood from a surface and molecular perspective, in particular not for base metallic NPs. This emphasizes the importance of profound physico-chemical characterization and elaboration of relevant test strategies. Together with experts in toxicology at Karolinska Institutet, Lund and Gothenburg universities, the team conducts research investigations to gain improved molecular and mechanistic understanding on how, and if, changes in surface reactivity, corrosion/oxidation processes and interactions with biomolecules on metallic materials at different relevant settings correlate with various acute and chronic toxic effects. The approach is highlighted for a selection of metals, alloys and metal oxides.
Jonas Hedberg is a Researcher and docent at the Division of Surface and Corrosion Science, KTH Royal Institute of Technology, Sweden. He has experience in corrosion, spectroscopy, and surfaces studies of interactions between nanoparticles and biological and environmental media.
Connections between natural organic matter adsorption, dissolution and electrochemistry for a selection of metal nanoparticles
Abstract: Environmental risk assessment of the diffuse dispersion of metal nanoparticles (NPs) requires knowledge on their environmental fate in terms of changes in particle characteristics and their transformation in contact with freshwater and similar media. This work describes how interactions with natural organic matter (NOM) and the electrochemical properties of metal NPs influence their dissolution and surface characteristics. Investigated NPs include copper, manganese, aluminum, tungsten carbide, tungsten carbide cobalt, and cobalt. NOM interactions were studied using dihydroxy benzoic acid (DHBA) and Suwannee River humic acid. For example, DHBA adsorbed via covalent bonding with NPs of copper and aluminum, which enhanced their degree of dissolution through weakening of the metal-oxygen bonds of the surface oxide. In all, this study shows that the comparison of NPs of different surface characteristics is a way to increase the knowledge on the environmental fate of NPs, useful in terms of improved understanding of dissolution mechanisms.
Rickard Arvidsson is an Associate Professor at the Dept of Technology Management and Economics, Chalmers University of Technology. He has an expertise in Life Cycle Assessment and Environmental Risk Assessment of nanomaterials.
Proxy measures for manufactured nanomaterials
Proxy measures are simple indicators able to provide early signs of environmental risk. Since environmental risk assessment of manufactures nanomaterials (MNMs) has proven challenging, the feasibility of using proxy measures was investigated. A literature review revealed a number of potential proxy measures for MNMs, which were further evaluated based on environmental relevance and data availability, resulting in a number of promising measures: production volume, release and a number of ecotoxicity measures. Two of these – production volume and short-term aquatic ecotoxicity – were employed in a proof-of-concept test for seven MNMs: titanium dioxide, cerium dioxide, zinc oxide, silver, silicon dioxide, carbon nanotubes and graphene. The proxy measures were feasible for a screening environmental assessment of these MNMs. The results from the test furthermore revealed that some of the MNMs had high production volume and one had high ecotoxicity, but none had both high production volume and ecotoxicity.
Anna Furberg is a PhD Student at the Dept. of Technology Management and Economics, Chalmers University of Technology, with an expertise in Life Cycle Assessment.
Societal flows of cemented tungsten carbide – the case of tire studs
Abstract: Cemented tungsten carbide (WC-Co) is applied in many different applications due to its properties of high hardness and toughness. Tire studs represent one such application and is used in many countries in order to increase the friction between the tires and the winter road. Previous studies have indicated emissions of WC-Co nanoparticles originating from tire stud use but the magnitude of these emissions remain unknown. This study conducted a material flow analysis for tungsten, which is the main constituent of the tire stud pins, and quantified WC-Co nanoparticle emissions from the use of tire studs in Sweden. The results show that 100% of the tungsten in tire studs become dissipated and that 0% is functionally recycled. A comparison of WC-Co nanoparticle emissions with emissions of some engineered nanomaterials in various applications in Sweden showed that the WC-Co nanoparticle emissions were larger than for e.g. nanosilver.
Zareen Abbas is an Associate Professor at the Dept of Chemistry and Molecular Biology, University of Gothenburg. He has expertise in modeling the interactions of nanoparticles with organic molecules in ionic solutions as well as nanoparticles interactions with artificial membranes. Analytical theories along with simulation methods such as Monte Carlo (MC) and Molecular Dynamics (MD) are used for modeling.
Modelling the Interactions of Organic Molecules with Nanoparticles
Abstract: Fate and transport of nanoparticles in aqueous media are influenced by many factors such as ionic composition, nature of organic matter present in the aqueous media and state of aggregation of nanoparticles. Due to the complexity of system a multilevel modelling approach is needed. In this presentation, it will be shown how information gained by density functional theory (DFT) about the deprotonation of organic molecules, surface charging of nanoparticles in different ionic solutions obtained by Corrected Debye-Hückel (CDH) theory can further be used in molecular dynamics (MD) simulations, to model the organic nanoparticles interactions with the charged nanoparticle surfaces. The modelling results are compared with the experimental results. Moreover, future direction of modelling work will be highlighted.
Åsa Boholm is a Professor at the School of Global Studies, University of Gothenburg. Her expertise is social science risk research.
Magnus Jansson is economics master, file. Dr in Psychology and Certified Psychologist. Magnus's research is primarily focused on decision making and financial assessments. He disputed 2011 at the University of Gothenburg with the dissertation "Psychological influences on adoption of socially responsible investment". The thesis examines the psychological motives and driving forces that influence investors to take ethical consideration into their investment decisions.
Frida Book is a PhD Student at Dept of Biological and Environmental Sciences, University of Gothenburg.
Toxicity screening of seven different types of commercial silica nanoparticles using cellular and organismic assays: importance of surface and size
Abstract: We show that seven different types of commercial, biocide-free, colloidal silica particles of nominal sizes between 7 and 100 nm with 3 different surface chemistries (Na-stabilized aluminized and silane-modified) are not toxic to the bacterium Pseudomonas putida, and the algae Raphidocelis subcapitata in the concentration range 5-500 mg/L. They are also not acutely toxic to Daphnia magna at concentrations up to 10 000 mg/L. Six silica particles are toxic to gill cell lines from Rainbow trout (Oncorhynchus mykiss), showing a clear concentration-response relationship with EC50 values between 13-92 mg/L. Toxicity increases with hydrodynamic sizes and is dependent on particle surface area. The average EC50 across the tested particles is 2.0 m2 (± 0.3 m2/L). Surface modifications clearly impact toxicity, with silane-modified particles being not cytotoxic.
Julián Alberto Gallego Urrea
Julián Gallego is a postdoctoral research fellow in the department of marine sciences and connected to the research group on marine environmental nanochemistry. His main research focus is in inorganic environmental aquatic chemistry with emphasis in analytical chemistry, colloidal chemistry and chemical modelling.
Fate of nanomaterials in Seawater: experimental approaches
Authors: Andreas Gondikas, Julián Gallego Urrea, Martin Hassellöv
Abstract: Due to the high ionic strength of seawater, which suppresses the electrostatic repulsive forces between particles, it is often perceived that nanomaterials are likely to aggregate and settle. However, large scale biogeochemical processes induce high variability to the chemical composition of these waters. Increased sunlight and temperature in the spring trigger a rapid growth of phytoplankton and microorganisms (spring bloom), which in turn triggers zoo plankton grazing and viral activity, leading to dissolved organic matter release through exudates, sloppy feeding, and cell lysis. Here we present lab-scale aggregation experiments using environmentally relevant concentrations (low ppb range) of model nanoparticles in seawater freshly collected from the Gullmarn fjord. The seawater composition in the spring and summer resulted in aggregation rates reduced by more than 60% compared to the winter seawater composition. Organic matter such as fibrils and exopolymeric substances were found to attach on the nanoparticles, thus stabilizing them in the high salinity water.
Joachim Sturve is an Associate Professor at the Department of Biological and Environmental Sciences, University of Gothenburg. The main aim of his work is to identify effects of, and track the presence of, pollutants in the environment. The research is focused on the field of aquatic toxicology and ecotoxicology in connection with physiological changes in aquatic organisms.
Begoña Espiña is the Leader of the Water Quality Research Group within the Department of Life Sciences at INL, International Iberian Nanotechnology Laboratory, Portugal. Dr. Espiña focuses her research on developing biosensors based on nanomaterials for capture of chemical contaminants in water as well as developing methods for nanomaterials’ fate, bioaccumulation and toxicity.
Evaluation of toxicity and bioaccumulation of commercial nanomaterials used in plastic industry in aquatic organisms.
Abstract: Last decade’s extensive development of novel nanomaterials have boosted a remarkable expansion potential for multiple industry sectors. Yet, current uncertainty in the regulatory framework, the limited literature available on their potential effects towards human health and environmental safety, and the scarce information on their specific properties have limited their use in the industry. Our research group has screened the in vivo toxicity in zebrafish embryos and, toxicity and bioaccumulation in mussels of different engineered nanoparticles that are already commercially incorporated in materials developed in the industrial sector of plastics improving their barrier, mechanical and/or conductive properties. This research is being developed in the framework of the funded project Interreg SUDOE-NanoDESK. NanoDESK aims to promote the investment in nanotechnology in a safe and sustainable way in the plastic sector, solving the current barriers by developing a set of tools to support decision making.
Dr Marco Monopoli is StAR Research Lecturer in the Department of Pharmaceutical and Medical Chemistry in the RCSI in Ireland, where he is establishing a multi-disciplinary centre focused to obtain a complete understanding of the mechanisms of interaction between nanomaterials and living systems essential for Nanomedicine, Nanotoxicology applications and to evaluate their Environmental Impact.
Understanding the nanomaterial interaction with biomolecules, a journey from the safety assessments to applications in modern medicine
Abstract: Nanotechnology is one of the primary drivers of technology innovation, and it is one of the leading pillars of the six Key Enabling Technologies of H2020. Among the different application scopes, its use in medicine has attracted considerable attention for its potential advances in healthcare, personalised medicine and to tackle complex issues such as the targeted and programmed delivery of drugs.
Because of their small size, they can directly interact with biomolecules in an entirely different way and their behaviour in biology is still not fully understood. Once in biological fluids, NPs rapidly interact with biomolecules from the environment that firmly and rapidly adsorb to the NP surface forming the long-lived biomolecular corona. [1,2] The biomolecular corona gives a new identity to NP in the biological milieu as it has been shown to interact with cellular receptors directly and can affect the journey as it travels through the body. [3,4]
It is now clear that these interactions lead to a dramatic surface changes and a new identity of the NP in biological fluid and the corona can induce unpredictable immunological responses and can hamper their therapeutic efficacy.
The protein corona is derived from proteins in biological fluids, many of which are glycosylated. We have now shown that the biomolecular corona has a strong glycosylation component that is biologically active and this class of biomolecules plays a dramatic role in the NP colloidal stability and firmly controls the NP biological fate and, if controlled, can offer new opportunities in nanomedicine 
 Monopoli MP, Aberg C, Salvati A, Dawson KA. Biomolecular coronas provide the biological identity of nanosized materials. Nature Nanotechnology. 2012;7:779-86
 Nel AE, Madler L, Velegol D, Understanding biophysicochemical interactions at the nano–bio interface et al Nature Materials, 2009, 8, 543-557.
 Salvati A, Pitek AS, Monopoli MP, Prapainop K, Bombelli FB, Hristov DR, Mahon E, Dawson KD. Transferrin-functionalized nanoparticles lose their targeting capabilities when a biomolecule corona adsorbs on the surface. Nat Nanotechnol. 2013;8:137-43.
 Maiolo D, Bergese P, Mahon E, Dawson KA, Monopoli MP. Surfactant Titration of the Nanoparticle-Protein Corona. Analytical Chemistry. 2014; 86, 12055–12063
 Wan S, Kelly PM, Mahon E, Stockmann H, Rudd P, Caruso F, Dawson KA, Yan Y, Monopoli MP*. The "Sweet" Side of the Protein Corona: Effects of Glycosylation on Nanoparticle-Cell Interactions. ACS Nano. 2015
Michael Persson, Ph.D., MBA, joined Nouryon (i.e. former AkzoNobel Specialty Chemicals) 1989 and have had several manager positions within the RD&I organization. He is since 2012 acting as innovation manager at Nouryon and adjunct professor in silica chemistry at Department for Chemistry and Chemical Engineering, Chalmers University of Technology. The focus areas have been material and product development based on submicron and/or nanoparticles often in close co-operation with institutes and universities.
Anna Lennquist holds a PhD in ecotoxicology from the University of Gothenburg, Sweden. She also holds a degree in journalism from the same university and has a strong interest in communicating scientific findings to a wider audience. Anna joined ChemSec in 2011. She has focused on substitution since then, first in the development of the SUBSPORT project, and since 2013 as project manager of the SIN List. 2016-2018 she was also in charge of developing ChemSec Marketplace.
Åsalie Hartmanis is managing director of SwedNanoTech, an association for promoting Swedish nanotechnology to national and international stakeholders. She has a long experience of strategic and operational communication on an executive management level from both public and private organizations, as well as media. During her entire career, she has been working in the interface between research and business development. Åsalie Hartmanis holds a Master’s degree in Biotechnology from the Royal Institute of Technology, Stockholm, Sweden.
Heike Hellmold is currently the head of the Unit for Toxicology Sciences at Karolinska Institutet. The unit has been an integral part of Swetox, an academic consortium of eleven universities with focus on collaboration in the area of chemicals, health and environment. She has a PhD in molecular toxicology and comprehensive experience of safety assessment of pharmaceuticals at AstraZeneca. During the past 3 years Heike has lead the startup of the Swedish National Platform for Nanosafety, SweNanoSafe and is also the Chair of the Cooperation Council for SweNanoSafe.
Lauge Peter Westergaard Clausen
Lauge Peter Westergaard Clausen is currently working as a postdoctoral fellow at the Technical University of Denmark. He has a PhD degree within plant uptake of soil- and groundwater pollutants but have lately moved into regulatory engineering, with focus on nanomaterial and plastic regulations in EU.
Simon Larsson, PhD in Social Anthropology, affiliated with Gothenburg Research Institute, School of Business, Economics and Law, University of Gothenburg. Has a research interest in regulation and governance of new technology, and in public and experts' perceptions of risk.
Eiwe Ljungblom is since more than thirty years an International Serial Entrepreneur and Business Angel, mostly in Sustainability. He is one of the initiators to TestBed Nano under construction. One other role is Founder and Executive Chairman of “Growth in Service of Mankind” - Tillväxt I Mänsklighetens Tjänst - who operates towards a Sustainable Market trough Open Innovation and Circular Economy for Sustainable Business Models. For example, he has also created Social Sustainable Economy through entering Generic Medicine in Swedish Welfare and Life Science.